Abstract:
Investigation of genetic diversity and molecular characterization in high yielding rice varieties
is important for their identification. The experiment was conducted during 2016 - 2017 to
analyse the genetic diversity of fifteen high yielding rice varieties in Bangladesh by using
random amplification of polymorphic DNA (RAPD) markers. Polymorphism was revealed
in 12 RAPD primers out of 30, whereas no other reaction was detected on the remaining 18
primers. The 40 out of 45 bands (88.89%) polymorphics were produced by the primers and
ranged from 50 to 100%. The maximum number of polymorphic bands was produced by the
primer OPB-18 whereas the lowest number of polymorphic bands belonged to OPC-12. The
genetic similarity coefficients were determined with the RAPD data, which ranged from 0.47
to 0.94. The unweighted paired group of arithmetic means (UPGMA) dendrogram presented
the studied rice varieties into two major clusters. Moreover, the value of Nei’s genetic diversity
is 0.26 and the Shanon information index is 0.41. The study produced distinct positions,
suggesting that the genotypes were different from each other. The results indicated that
these markers could be efficient for comparing the genetic relationships, patterns of variation,
and measurement of genetic distance among rice varieties. Considering all of these results,
RAPD analysis is found to be an effective tool for estimating the genetic diversity of different
rice varieties. The outcomes of this research may contribute to the germplasm data of rice
accessions and a future breeding program of rice genotypes.
